脑平康颗粒对短暂脑缺血后大鼠学习记忆能力的影响

Effects of Naopingkang Granule on Learning and Memory in Rats with Transient Cerebral Ischemia

目的:观察脑平康颗粒对短暂脑缺血后大鼠学习与记忆能力的影响。方法:将Wistar大鼠随机分成6组:对照组、短暂脑缺血模型组、脑平康颗粒高、中、低剂量组(12,6,3 g.kg-1)和多奈派齐(5 mg.kg-1)。ig给药,每日1次。采用两侧颈总动脉夹闭加放血性低血压法制作大鼠短暂性全脑缺血模型,利用Morris水迷宫法和避暗法观察脑平康颗粒对短暂脑缺血后大鼠学习与记忆能力的影响,并检测海马乙酰胆碱酯酶(AchE)活性。结果:Morris水迷宫实验,脑平康颗粒显著抑制短暂脑缺血大鼠逃避潜伏期、显著延长撤台后大鼠在目标象限的时间(P<0.05);避暗实验,脑平康颗粒显著抑制短暂脑缺血大鼠潜伏期(P<0.05)且与镇痛作用无关;脑平康颗粒显著抑制海马AchE活性(P<0.05)。结论:脑平康颗粒对短暂脑缺血后大鼠学习与记忆能力有明显改善作用,其作用机制可能与海马胆碱的代谢有关。

Objective：To investigate the effects of Naopingkang granule on learning and memory in rats after transient cerebral ischemia.Method：Sixty Wistar rats（n=10/group） were randomly divided into six groups：the normal goup,the model group,the Donepezil group,high Naopingkang granule group（12 g·kg^-1）,middle Naopingkang granule group（6 g·kg^-1） and low Naopingkang granule group（3 g·kg^-1）.The treament groups were orally administered once per day separately,whereas the normal and model group were orally administered with distilled water.Ischemia model was made with 2-vessel-occlusion＋hypotension,Morris water maze test and step-through passive avoidance test were employed to investigate the influences of Naopingkang granule on transient cerebral ischemia in rats.Result：In Morris water maze test,Naopingkang granule could significantly decrease escape latency time,swimming times in target quadrant was significantly recovered compared with that of model group（P〈0.05）;in Step-through passive avoidance test,the shorter step-through latency and much error number induced in transient cerebral ischemia was significantly reversed by Naopingkang granule treatment compared with that of model group（P〈0.05）;activety of AchE was inhibited compared with that of model group（P〈0.05）.Conclusion：Naopingkang granule could improve learning and memory.