罗布麻叶中黄酮类成分的定位与相对定量

Location and relative quantity of flavonoids in the leaf of Apocynum venetum

为探讨罗布麻不同生长年限植株上不同部位叶中黄酮类成分的分布与相对含量变化,利用激光共聚焦显微技术对7月份采收的罗布麻1～3年龄植株上部、中部、下部的叶中黄酮类成分进行组织定位和相对定量。所记录数据经正态性、方差齐性检验后进行方差分析(ANOVA),并使用LSD检验作多重比较。结果显示黄酮类成分主要分布在叶脉维管束木质部导管壁、叶脉上下方厚角组织、表皮细胞及外方蜡质层。统计分析表明罗布麻叶中黄酮类成分的含量在不同年龄植株上不同部位之间有一定的差异。

In this study,laser scanning confocal microscopy（LSCM） was used to determine the location and relative quantity of flavonoids in the leaves of Apocynum venetum L.from the top,middle and basal parts of the branch.The leaves of the plants of one,two and three years old,separately,were collected in July.ANOVA and LSD test were employed in the statistical analysis.The results indicated that flavonoids located mainly in xylem conduit of vein,collenchyma,epidermic cells and cuticle.The data of flavonoids contents under statistical analysis showed that difference existed in the leaves of different parts and different ages.This study provided the reliable scientific material about the analysis of the ecological and the exploitation of the leaves of Apocynum venetom L.