食管鳞癌间隙连接蛋白Cx32和Cx43的检测及意义

Determination and significance of connexin 32 and 43 in esophageal squamous cell carcinoma

目的检测间隙连接蛋白(connexin,Cx)32和43在食管鳞癌的表达,探讨其在食管鳞癌发生、发展中的作用及临床意义。方法用免疫组织化学技术(Elivision法)检测78例食管鳞癌组织和22例癌旁食管鳞状上皮组织中Cx32和Cx43的表达情况。结果癌旁食管鳞状上皮中Cx32和Cx43主要表达于细胞膜,阳性表达率均显著高于癌组织。Cx32和Cx43在食管鳞癌细胞膜表达与组织的分化程度、浸润深度、淋巴结转移及临床分期均有一定相关性,而与性别、年龄和病变部位无关。结论 Cx32和Cx43的表达随着食管鳞癌病理分级的增高而降低,其异常表达可能是食管鳞癌发生、发展的重要因素之一。

Objective To determine the expression of connexin 32（Cx32） and 43（Cx43） in esophageal squamous cell carcinoma（ESCC） and investigate their effects and clinical significance in the occurrence and development of human ESCC.Methods The expressions of Cx32 and Cx43 in 78 cases of ESCC and 22 cases of adjacent normal tissues were determined by Elivision immunohistochemical technique.Results Cx32 and Cx43 were typically expressed in the membrane of normal esophageal squamous epithelium,and the positive rate of Cx expression were significantly higher in normal esophageal tissues than that in cancer tissues（P0.01）.In ESCC,the expression of Cx32 and Cx43 was closely correlated with the differentiation grade,invasive depth,metastasis of lymph nodes and clinical staging,but not related with sex,age and lesion distribution.Conclusions The expression of Cx32 and Cx43 decreased with the elevated pathologic grade of ESCC.The abnormal expression may be considered as an important factor in the occurrence and development of ESCC.