摘要
目的探讨高分子聚合物是否能逆转或减轻血浆诱导的肺表面活性物质(PS)失活。方法应用闭泡式表面张力仪分别测定高分子聚合物葡聚糖(Dextran)、聚乙二醇(PEG)和透明质酸(HA)、PS及加入了1%~3%血浆的混合物的最大表面张力(γmax)和最小表面张力(γmin),并计算其稳定系数(S.I.)。结果不同的高分子聚合物、血浆及其混合物的γmin均大于10 mN-m,S.I.小于0.8。PS(0.84 g·L-1)的γmin及S.I.分别是(1.78±0.03)mN-m和1.71±0.01。当PS与1%、2%、3%血浆混合后,其混合物的γmin分别上升为(21.96±0.15)mN-m,(22.31±0.34)mN-m和(28.02±1.36)mN-m;S.I.下降为0.14±0.03,0.12±0.01和0·36±0·03;再加入5%PEG,5%Dextran或0.25%HA后,其γmin分别下降为(1.60±0.04)mN-m,(1.35±0.06)mN-m,(18.66±0·73)mN-m;(1.13±0.01)mN-m,(1.28±0.01)mN-m,(16.68±0.31)mN-m和(1.16±0.01)mN-m,(1.22±0.01)mN-m,(1.23±0·01)mN-m。S.I.分别为1.76±0.01,1.77±0.01,0.31±0.03;1.82±0.01,1.80±0.00,0.46±0.03和1.82±0.01,1.81±0.00,1.81±0.01。PEG(0%、2.5%、5.0%、10.0%)+PS+血浆(2%)组γmin分别为(22.32±0.34)mN-m,(1.09±0.01)mN-m,(1.35±0.06)mN-m,(0.96±0.02)mN-m;Dextran(0%、2.5%、5.0%、10.0%)+PS+血浆组γmin分别为(22.32±0.34)mN-m,(2.03±0·04)mN-m,(1.28±0.01)mN-m,(1.10±0.09)mN-m;HA(0%、0.125%、0.250%、0.500%)+PS+血浆组γmin分别为(22.32±0·34)mN-m,(9.60±0.20)mN-m,(1.22±0.01)mN-m,(0.92±0.01)mN-m。结论在体外实验中,国产PS具有很好的表面活性;高分子聚合物(PEG、Dextran、HA)可以逆转血浆引起的PS失活;与PEG和Dextran比较,HA和PS混合,对抗血浆引起的PS失活效果更好。
Objective To verify whether plasma-induced pulmonary surfactant(PS) inactivation may be prevented or reduced by polymers.Methods The maximum surface tension(γmax) and minimum surface tension(γmin) of nonionic polymer dextran,polyethylene glycol(PEG),ionic polymer hyaluronan(HA),PS,plasma and their mixtures by captive bubble surfactometer were measured,respectively.Their respective stability index(S.I.) was calculated.Results The minimum of the different polymers,plasma and their mixtures were more than 10 mN-m and the S.I.were less than 0.8.The γmin and S.I.of PS(0.84 g·L-1) were(1.78±0.03) mN-m and 1.71±0.01,respectively.Different of polymers significantly enhanced the ability of PS to lower the γmin and increased S.I.in presence of plasma.The γmin and S.I.of PS mixed with 1%,2%,3% plasma were(21.96±0.15) mN-m,(22.31±0.34) mN-m and(28.02±1.36) mN-m;0.14±0.03,0.12±0.01 and 0.36±0.03,respectively.When mixed with 5% PEG,5% Dextran or 0.25% HA,the γmin decreased to(1.60±0.04) mN-m,(1.35±0.06) mN-m,(18.66±0.73) mN-m;(1.13±0.01) mN-m,(1.28±0.01) mN-m,(16.68±0.31) mN-m and(1.16±0.01) mN-m,(1.22±0.01) mN-m,(1.23±0.01) mN-m,respectively,and the S.I.were 1.76±0.01,1.77±0.01,0.31±0.03;1.82±0.01,1.80±0.00,0.46±0.03 and 1.82±0.01,1.81±0.00,1.81±0.01,respectively.The similar effect were seen when different concentrations of polymers with PS and plasma.The γmin of PEG(0%,2.5%,5.0%,10.0%)+PS+plasma(2%) group were(22.32±0.34) mN-m,(1.09±0.01) mN-m,(1.35±0.06) mN-m,(0.96±0.02) mN-m,respectively;the γmin of Dextran(0%,2.5%,5.0%,10.0%)+PS+plasma group were(22.32±0.34) mN-m,(2.03±0.04) mN-m,(1.28±0.01) mN-m,(1.10±0.09) mN-m,respectively;the γmin of HA(0%,0.125%,0.250%,0.500%)+PS+plasma group were(22.32±0.34) mN-m,(9.60±0.20) mN-m,(1.22±0.01) mN-m,(0.92±0.01) mN-m,respectively.Conclusions The PS made in China has good ability to reduce the surface tension.Nonionic and ionic polymers can reverse inactivation of surfactant induced by plasma in vitro.HA may be more effective than PEG and Dextran when mixed with PS.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第14期1115-1118,共4页
Journal of Applied Clinical Pediatrics
基金
北京自然科学基金(7042018)