毛木耳多糖的分析研究

The Determination of Polysaccharide in Auricularia Polytricha(Mont)Sacc

用云南产市售的毛木耳子实体经提取纯化后得到的纯毛木耳多糖精品（简称ＰＡＡ），采用聚丙烯酰胺凝胶电泳法检验其纯度，已符合定性定量分析标准。经ＵＶ 法测定于２６０ｎｍ 和２８０ｎｍ 无特征吸收峰，经ＣＨＮ 元素分析仪测定不含氮。说明ＰＡＡ 中无核酸及蛋白质。ＩＲ扫描有特征多糖吸收峰；分子量测定约为１２ 万，总含糖量为６１．２９％ ，糖醛酸含量为１５．２４％ 将ＰＡＡ 经水解后用硅胶Ｇ 薄层层析和气相色谱分析，其单糖组成为Ｌ－ 岩藻糖，Ｌ－ 阿拉伯糖，Ｄ－ 木糖，Ｄ－ 甘露糖，Ｄ－ 葡萄糖及葡萄糖及葡萄糖醛酸。上述单糖的摩尔含量比分别为０．０８∶０．０４２∶０．１８∶３．００∶１．２０。原子吸收光谱分析说明，ＰＡＡ 中含有多种对机体有益的微量元素。

The polysaccharide was extracted from auricularia polytricha(mont)sacc. The UV, IR, elementary analysis and gel electrophoresis have expressed that purity of polysaccharide was reagent grade and there weren’t nucleic acid and protein in polysaccharide. It’s molecular weight is about 1.2×10 5, total sugar is 61.29%. The component of monosaccharide are L-fucose, L-arabinose, D-xylose, D-mannose, D-glucose and glucuronic acid, which mole ratio are 0.08∶0.042∶0.18∶3.00∶1.2,respectively. Some trace element were also discovered in auricularia polytricha(mont)sacc.