PAHs相关肺癌患者血浆GSH-Px/MDA、P21蛋白水平的研究

Study of Serum GSH-Px,MDA and P21 Protein in Lung Cancer Patients Induced by PAHs

目的探讨多环芳烃(Polycy clic aromatic hydrocarbons,PAHs)相关肺癌患者血浆谷光甘肽(GSH-Px)、丙二醛(MDA)、P21蛋白水平与氧化应激的关系,为PAHs相关肺癌发病机制及诊断指标的研究提供参考依据。方法实验对象分为PAHs相关肺癌者(肺癌组31人)、非肿瘤性肺病者(配比组34人)和健康者(健康组33人),采用二巯代硝基苯甲酸比色法(DTNB法)、硫代巴比妥法(TAB法)、蛋白质印迹法(Western Blot)分别检测受试者血浆中GSH-Px活力、MDA含量及P21蛋白水平。结果血浆中GSH-Px活力呈现肺癌组<配比组<健康组的梯度(P<0.05);与配比组和健康组比较,肺癌组MDA含量及P21蛋白水平明显增高(P<0.05)。在肺癌组中,MDA含量及P21蛋白水平与GSH-Px呈负相关(分别为r=-0.2269,P<0.01和r=-0.1517,P<0.05),P21与MDA呈正相关(r=0.2539,P<0.01)。结论 PAHs相关肺癌可能与氧化应激密切相关;联合检测GSH-Px、MDA、P21蛋白水平可能有助于PAHs相关肺癌的早期诊断。

Objective To explore the relationship between serum levels of glutathione peroxidase（GSH-Px）,malonydialdehyde（MDA）and P21in PAHs induced lung cancer and its possible incidence mechanism and diagnostic criteria.Methods DTNB,TAB and Western blot were used respectively to detect the serum level of GSH-Px,MDA and P21 protein in patients of lung cancer group（31 people）,matched group（34 people）and healthy group（33 people）.Results Results of GSH-Px activity showed in the order of lung cancer groupmatched grouphealthy group,the differences were Significant（P0.05）.The levels of MDA and P21 protein were significantly higher in cancer group than that in matched and healthy group（P0.05）.In the cancer group,the level of GSH-Px was negatively correlated with MDA and P21（r=-0.1520,P0.05 and r=-0.1517,P0.05）and positively correlated with serum levels of MDA and P21（r=0.2539,P0.01）.Conclusions Oxidative stress might be closely associated with PAHs induced lung cancer.Combined detection of GSH-Px,MDA and P21 might be helpful for the early diagnosis of PAHs induced lung cancer.