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BST-2抑制HIV-1病毒样颗粒释放的研究

Inhibition of HIV Virus-like Particles Production by BST-2
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摘要 BST-2是最近发现的可以抑制成熟HIV-1(human immunodeficiency virus,HIV)病毒颗粒从哺乳动物细胞表面释放的宿主因子,随之发现其也可以抑制多种包膜病毒的释放。本研究采用密码子优化的表达HIV-1 gag和gag-pol蛋白的质粒所形成的病毒样颗粒作为研究对象,观测BST-2对这两种病毒样颗粒(Virus-like particle,VLP)的释放抑制情况及其作用机制。结果发现,瞬时表达和稳定表达的BST-2均可以显著抑制病毒样颗粒从哺乳动物细胞释放,同时发现这两种病毒样颗粒(gag/gag-pol)的释放都可以被BST-2抑制;而且,HIV-1中Vpu蛋白可以拮抗BST-2抑制HIV病毒样颗粒释放的作用,另外,通过化学试剂和酶学方法处理,确证BST-2可以被包装进病毒样颗粒中。 Recently,BST-2 has been identified as an effective cellular factor that prevents the release of human immunodeficiency virus type 1 and other enveloped viruses by tethering virus particles to the cell surface.Here,we showed that the production of HIV-1 virus-like particles was markedly inhibited by BST-2.Both the transient and stable expressing of BST-2 had the same function and Vpu rescued the release of HIV-1 VLP in the presence of human BST-2.Consistent with a direct tethering mechanism,we confirmed that proteolysis releases restricted virions and further showed that this removed the ectodomain of BST-2 from the cell surface.
作者 杜应莲 胡斯奇 庞晓静 岑山 金奇 郭斐
机构地区 中国医学科学院北京协和医学院病原生物学研究所病毒基因工程国家重点实验室 中国医学科学院北京协和医学院医药生物技术研究所免疫生物室
出处 《病毒学报》 CAS CSCD 北大核心 2011年第4期319-325,共7页 Chinese Journal of Virology
基金 艾滋病和病毒性肝炎等重大传染病防治科技专项(2009ZX10004-303)
关键词 BST-2 人类免疫缺陷病毒 细胞限制因子 病毒释放 BST-2 HIV Cellular restriction factor Virus release
分类号 R373.9 [医药卫生—病原生物学] Q78 [生物学—分子生物学]
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参考文献15

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病毒学报
2011年 第4期

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