厌氧产甲烷受抑制过程的三维荧光光谱解析

Analysis of the EEM Fluorescence Spectra of Effluent from Anaerobic Methane Reactor during the Inhibition Process

应用三维荧光光谱表征了厌氧产甲烷反应受抑制前后的出水,采用平行因子分析方法分析,得到各主成分的激发发射光谱图以及相对浓度。分析结果显示,运行正常的产甲烷反应器出水以蛋白质、辅酶NADH和F420为主;产甲烷反应受到抑制后,甲烷生成速率迅速下降,同时辅酶F420荧光消失,反应器出水中以蛋白质和辅酶NADH为主。样品稀释后,蛋白质主成分的荧光强度得分与化学分析结果显著相关,相关系数为0.93;辅酶F420的相对浓度与甲烷产生速率成线性相关,表明辅酶F420可以表示特定生物反应器的产甲烷活性。该研究提供了一种快速表征产甲烷反应器运行状态方法。

The effluents from the anaerobic methane reactor were characterized by the EEM fluorescence spectra.The EEM fluorescence spectra and relative concentration of the main components were acquired by PARAFAC models.Results show that the main components of the effluents from the uninhibited reactor were protein,coenzyme NADH and F420.When the methane-producing reaction was inhibited,the production rate of methane decreased rapidly.Correspondingly,the fluorescence of F420 disappeared and the main components of the reactor were protein and NADH.The fluorescence intensity scores of protein from the diluted sample were remarkably correlated to the protein determination by chemical analysis and the correlation efficient was 0.93.The fluorescence intensity scores of F420 were correlated to the production rate of methane,which implicated that coenzyme F420 could be used to reflect the activity of methane production in a certain bioreactor.This work provides a new method to monitor the operation status of anaerobic methane-producing reactor.