牛乳β-乳球蛋白热稳定与免疫原性关系的光谱学研究

Spectroscopic Studies on the Relationship Between Heat Treatment Process and Immunogenicity of β-Lactoglobulin in Milk

利用圆二色性光谱和荧光光谱对牛乳β-乳球蛋白的热变性过程进行光谱学分析,得到其热变性过程的光谱学特征;结合生物信息学方法,进一步分析了热变性过程中牛乳β-乳球蛋白构象变化的特点,探讨了热变性使牛乳β-乳球蛋白蛋白构象变化导致其免疫原性发生变化的关系,建立了一种研究过敏原蛋白热变性与免疫原性关系的光谱实验方法,为探讨热变性改变食物中主要过敏原的免疫原性提供了理论依据。

The thermal denaturation of β-lactoglobulin of milk was investigated by using DSC,circular dichroism spectra,and fluorescence spectra systematically.And its corresponding immunogenicity was tested with ELISA experiments.In addition,bioinformatics methods were also used to explain the spectral characteristics and its corresponding immunogenic change,analyze the conformational changes of the protein during thermal denaturation,and discuss the relationship between conformational change of β-lactoglobulin caused by heat and immunogenic change.Results from this study should be useful to the establishment of a spectral method examining the extent of thermal denaturation of the food allergen,and be helpful to the understanding of the mechanism of immunogenic change of food allergen treated by heat.