摘要
采用反相高效液相色谱-二极管阵列检测法(RP-HPLC-PAD)对槟榔花3种提取物中多酚类化合物的色谱分析条件进行优化,分别探讨流动相的组成、流动相中醋酸浓度、醋酸与甲醇的比例和柱温对保留时间的影响,确定梯度分离条件,并对3种不同槟榔花提取物中多酚类化合物进行定量分析。结果表明,3种提取物中均含没食子酸、香豆酸、表儿茶素、阿魏酸、芦丁和柚皮素,其中表儿茶素、没食子酸和香豆酸的含量相对较高。
The separation conditions of chromatography were optimized with a reversed-phase high performance liquid chromatographic photodiode array detection（RP-HPLC-PAD）method for the determination of nine polyphenols in three Areca infloreseences. The relationship of glacial acetic acid concentration in mobile phase and the retention time, the effects of mobile phase composition, flow phase and the proportion of methanol were discussed respectively. Then the influence of gradient separation conditions was established. At the same time, three Areca inflorescenees extracts of phenolic compounds were quantitatively analyzed. The results showed that the three extracts all contained gallic acid, eoumalic acid, epicateehin, fumalie acid, naringenin and rutin, in which the contents of epicatechin, gallic acid and coumalic acid were higher than others
出处
《热带作物学报》
CSCD
2011年第5期965-969,共5页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金(No.310101)
海南省重点科技计划项目(090138)资助
