龟鹿二仙胶及其拆方对SD大鼠及豚鼠软骨细胞增殖的比较研究

Comparative Effect of Guilu Erxian Glue and its Decomposed Recipes on Chondrocyte Proliferation of SD Rat and Cavia Cobaya

目的:通过观察龟鹿二仙胶汤及其拆方对SD大鼠及豚鼠软骨细胞增殖的影响,比较在生理及病理状况下龟鹿二仙胶汤及拆方各药物的作用。方法:取1月龄SD大鼠和4月龄豚鼠膝关节软骨建立软骨细胞体外培养体系,运用MTT法比较龟鹿二仙胶及其拆方含药血清对不同软骨细胞增殖的影响。结果:中药血清各组对SD大鼠软骨细胞增殖均具有非常显著的促进作用,西药组明显高于中药各组;龟板与全方效果相当。中药血清各组对豚鼠软骨细胞增殖均具有非常显著的促进作用。龟鹿二仙胶、龟板促增殖效果与西药相比差异无统计学意义。人参的促增殖作用也比作用于正常软骨细胞时有所增强。结论:在病理状态下,龟鹿二仙胶、龟板的促增殖作用明显增强,人参在细胞机能状况下降时才明显发挥补虚作用。

Objective：To compare the effect of Guilu Erxian glue and its decomposed recipes on chondrocyte proliferation of SD rat and cavia cobaya,and to compare the role under physiological and pathological conditions.Methods：Using chondrocyte from 1 month SD rat and 4 months cavia cobaya established chondrocyte cultured system in vitro,and measured the degree of proliferation by MTT method after treating with different drugs.Results：Drug serum can promote the proliferation degree of chondrocyte in SD rats significantly.The effect of GHC group was highest,and the tortoise plastron glue group was as more as GuiLu ErXian glue group.Drug serum can also promote chondrocyte proliferation of cavia cobaya significantly.There was no significance among GuiLu ErXian glue group,tortoise plastron glue group,and the GHC group.And the promoting proliferation of ginseng was enhanced than normal chondrocyte.Conclusion：The proliferation of chongdrocyte is strongly promoted by GuiLu ErXian glue and tortoise plastron glue under pathological condition.And ginseng can play reinforce deficiency role when the cell function is declined.