80份甘蔗种质RAMP标记遗传多样性分析

Genetic Diversity of 80 Sugarcane Germplasm Using RAMP Markers

采用RAMP分子标记技术对80份甘蔗种质（32份祖亲种、48份栽培品种或品系）的遗传基础进行了分析。从30对引物组合中筛选出4对多态性较强引物,构建了甘蔗80份种质的RAMP指纹图谱,这4对引物组合共扩增出84条带,其多态性为91.7%。80份种质的遗传相似系数变化范围在0.433~0.988,平均0.710。聚类分析表明,随着相似系数结合线的不同,可分别将参试的甘蔗种质从属间（甘蔗属与斑茅种）、野生种（割手密种、大茎野生种、印度种、中国种）与栽培种（热带种）间、栽培种与杂交栽培品种（或品系）间区别开来。各祖亲种与杂交栽培品种（或品系）的遗传相似性由大到小依次为热带种〉印度种和中国种〉大茎野生种〉云南割手密种〉其他割手密种〉斑茅。另外,本试验首次利用RAMP标记,获得了部分热带种、野生种及斑茅种特异片段,并发现这些特异片段能不同程度地在具有其血缘的栽培种中得到传递。

80 accessions of sugarcane germplasm（32 parental species,48 cultivars or clones） were analyzed by random amplify microsatellite polymorphisms（RAMP） markers.4 pairs of primers were selected from 30 pairs,and used to establish the RAMP fingerprints of the germplasm,which amplified 84 polymorphic bands,and were accounting for 91.7%.80 accessions were clustered with RAMP data using UPGMA method.The genetic similarity coefficients among 80 germplasm ranged from 0.433 to 0.988 with a mean of 0.710.Based on the difference of the similarity coefficients,the sugarcane germplasm could be distinguished among intergenus（Saccharum and E.arundinaceus）,wild species（S.spontaneum,S.barberi,S.sinense and S.robustum）,cultivated species（S.officinarum）.cultivated species and cultivars derived from interspecific hybridization.The similarity between cultivars and parental species S.officinarum were the highest,and followed by S.barberi,S.sinense,S.robustum,S.spontaneum and E.arundinaceus in the order from high to low.Clustering based on RAMP showed that the groupings of most cultivars were corresponded to their pedigrees in which a given cultivar and one of its parents was always clustered into the same group.In the meantime,we found some specific DNA fragments of S.officinarum,wild species of Saccharum spp.and E.arundinaceus,which could be recovered in their derived cultivars with different probabilities.