基于SRAP标记的大花蕙兰种质资源遗传多样性分析

Genetic Diversity Analysis of Hybrid Cymbidium′s Germplasm Resources Based on SRAP Markers

采用SRAP技术分析了来源于不同国家和地区的42个大花蕙兰品种及4个国兰原生种之间的遗传亲缘关系。34对引物组合中筛选出29对带型稳定、多态性较好的引物组合,共扩增出398条谱带,其中387条为多态带,多态性比率97.2%,平均每个引物组合扩增多态性带13.3条。46份种质之间的相似系数变化范围为0.60-0.99,平均为0.76。基于29个SRAP标记的扩增结果,利用NTSYS 2.10e软件计算Dice遗传相似系数,并建立UPGMA聚类图,结果表明：在遗传相似系数0.69处将供试材料分为4类,较好地揭示了大花蕙兰品种及国兰原生种间的遗传多样性与亲缘关系,可为大花蕙兰种质资源利用及杂交育种中亲本选择提供科学依据。

SRAP was used to analyse the genetic relationships of 42 Hybrid cymbidium cultivars from different countries,and 4 species of native Cymbidium.29 stable and polymorphic primers were screened from 34 primers,and a total of 398 DNA bands were amplified,387 of which were polymorphic,the ratio of polymorphic bands was 97.2%.The average number of polymorphic DNA bands amplified by each primer was 13.3.The genetic similarity among 46 germplasm ranged from 0.60～0.99,with an average of 0.76.According to the amplification of 29 markers,genetic similarity coefficient of dice was calculated using software NTSYS 2.10e,and a dendrogram of genetic relationship was constructed using UPGMA method.The dendrogram showed that all the tested cultivars and species were classified into four cluster groups with the similarity coeficient of 0.69,and well revealed the genetic diversity and genetic relatives among Hybrid cymbidium cultivars and native species.The findings of this research would provide a scientific basis for Hybrid cymbidium germplasm utilization and parental selection in cross breeding.