大铃棉中棉所48主要经济性状的QTL定位分析

QTLs Location of Economic Traits for CCRI 48 with Big Bolls

利用4961对SSR引物对中棉所48的2个亲本进行多态性筛选,结果筛选到71对条带清晰、稳定性好的多态性引物,利用这些引物对261个F2群体进行扩增构建连锁图,获得了包含有49个标记位点的14个连锁群,共覆盖498.7 cM,约占棉花总基因组10.0%的遗传图谱。采用WinQTL Cartographer 2.5的复合区间作图法(C IM),对F2群体的铃重、衣分及纤维品质进行分析,共检测到11个稳定的QTLs,其中铃重2个、衣分4个、纤维整齐度2个、纤维细度2个、纤维伸长率1个。这些稳定表达的QTLs能解释较大的表型变异,可用于纤维品质及产量性状的标记辅助选择,也可为大铃、优质棉的分子标记辅助选择,改良、提高大铃基因的选择效率,提供理论依据。

4961 pairs of different sources SSR primers were used to screen polymorphism between two parents.71 pairs of them with stable amplification and clear bands were used to amplify among the 261 F2 and resulted into 71 polymorphism loci.Using the Joinmap 3.0 software with LOD of 3.0,49 loci was grouped into 14 linkage groups and covered 498.7cM,approximately 10.0% of the total recombinational length of the cotton genome.13 groups had been anchored to the chromosomes.A total of 11 stable QTLs,2 for boll weight,4 for lint percentage and 5 for fiber quality including 2 for fiber uniform,2 for micronaire and 1 for fiber elongation,were detected in F2 population of CCRI48 by Complex Interval Mapping（CIM）method with the software of Win QTL Cartographer 2.5.These stable QTLs with greater phenotypic variations could be used for maker assisted selection for yield and fiber quality and be used as molecular marker assisted breeding.