畜禽养殖污水中高效氨氮降解菌的筛选、鉴定及生长条件研究

Screening and identifying for an efficent strain of ammonia-nitrogen degrading bacteria from sewage and its growing conditions

从养殖污水中分离纯化得到1株高效氨氮降解菌,对其进行形态特征、生理生化、16S rDNA序列分析以及最佳生长条件研究,并将菌株投入养殖污水降解污水中的氨氮。结果表明:AN4菌株在NH4+-N初始质量浓度为50 mg/L的条件下,24 h内的氨氮降解率为92.5%;初步鉴定该菌株为苍白杆菌属(Ochrobactrum sp.),菌株的16S rDNA序列在Gen-Bank的登录号为GU345782;AN4菌株在装液量为60.64 mL,pH为7.06,葡萄糖为6.0 g/L的条件下培养,菌株的降解率可以达到94.28%;菌株对养殖污水中氨氮的降解率为12.3%。苍白杆菌菌属能够降畜禽养殖污水中的氨氮还未见报道,AN4菌株的筛选获得为生物降解养殖污水中的氨氮又提供了一种新型菌株。

An efficient strain in degarading ammonia-nitrogen AN4 was obtained with screening from sewage by its characteristics in morphology,physiology,16S rDNA gene sequence and the best growing conditions.Results indicated that the degrading efficiency of AN4 strain per 24 h for NH＋4-N was 92.5% of its initial concentration in 50 mg/L.The strain was preliminarily identified as Ochrobactrum sp.and the accession No.of its 16S rDNA gene sequence in GeneBank was GU345782.The optimal degradation conditions of AN4 in liquid culture were： culture medium 60.64 mL,pH 7.06,glucose 6.0 g/L.By which,the highest degradation rate of the AN4 reached to 94.28% and the reduction of NH＋4-N in sewage was 12.3%.It was first reported that Ochrobactrum sp.is capable in degrading ammonia-nitrogen and can be further used as a new strain for the biodegradation of ammonia-nitrogen in sewage.