维生素D_3对大鼠血清铁和肝脏铁调素基因表达的影响

Effect of vitamin D_3 on the expression of hepatic hepcidin mRNA and serum iron in rats

目的探讨维生素D3对大鼠体内铁代谢的影响。方法雌性SD大鼠12只,随机分成2组:实验组和对照组。实验组给予肌肉注射维生素D3 3×105 U/Kg,每周一次,持续4周;对照组按相同方法给予肌肉注射生理盐水。用全自动生化分析仪测大鼠血清钙、铁水平,用一步法逆转录聚合酶链反应方法测定肝脏铁调素(hepcidin)的mRNA水平。结果对照组大鼠血清钙、铁水平分别为2.48±0.03mmol/L和58.06±4.14umol/L;实验组大鼠血清钙、铁水平分别为3.01±0.12mmol/L和51.02±5.31umol/L;与对照组相比,实验组大鼠血清钙显著升高(P<0.01),血清铁显著降低(P<0.05),肝脏铁调素mRNA水平无显著变化。结论维生素D3可能通过增加肠道对钙的吸收降低了血清铁。

Objective To investigate the influence of vitamin D3 on the iron metabolism in rats in vivo. Methods Twelve female SD rats were divided into 2 groups： the experimental group and the control group. The rats in the experimental group were given vitamin D3 3×10^5 U/Kg by intramuscular administration once a week for 4 weeks. The rats in the control group were given normal saline by intramuscular administration. Automatic biochemical analyzer was used to measure serum iron and calcium levels. The expression of hepcidin mRNA in the liver were detected with one-step reverse transcription - polymerase chain reaction （RT-PCR）. Results The levels of serum calcium and iron were 2.48±0.03mmol/L and 58.06±4. 14umol/L, respectively in rats of control group, and 3. 01±0. 12mmol/L and 51.02±5.31umol/L, respectively in rats of experimental group. Serum calcium increased （P 〈0. 01 ） and serum iron （P 〈0.05） decreased in rats of experimental group significantly than those in control group. Expression of hepcidin mRNA in the liver did not change significantly. Conclusion Vitamin D3 may decrease serum iron level by increasing intestinal calcium absorption.