鼻咽癌颈淋巴结转移EB病毒基因的检测

Detection of Epstein Barr virus gene in nasopharyngeal carcinoma with neck lymphatic metastasis

目的 观察鼻咽癌(NPC)颈淋巴结转移EB病毒基因情况。方法 采用细针穿刺结合聚合酶链反应(PCR)技术对50例颈部肿块穿刺液进行EBVDNA检测。结果 30例鼻咽癌颈淋巴结转移者EBVDNA阳性数27例,阳性率为900%。7例恶性淋巴瘤者EBVDNA阳性数1例,阳性率143%。8例非NPC颈部转移癌者EBVDNA阳性数1例,阳性率为125%。5例鼻咽部炎症伴颈淋巴结增生者均未检测到EBVDNA。结论 细针穿刺结合PCR方法快速灵敏,所取标本量少,特异性高,尤其对于临床难以确认的隐匿性鼻咽癌及原发灶不明的颈部转移癌,具有一定的临床诊断价值,值得推广应用。

Objective To investigate the presence of Epstein Barr virus(EBV) gene in neck lymphatic metastasis of nasopharyngeal carcinoma.Methods Fifty patients′ masses in the neck were punctured and the fluids were analyzed with PCR for EBV DNA.Results Twenty seven cases showed EBV DNA positive among thirty cases of nasopharyngeal carcinoma with neck lymphatic metastasis, and the positive rate was 90%. One case was EBV DNA positive in seven cases of malignant lymphoma and the positive rate was 14.3% .One case was EBV DNA positive in eight tumor patients without NPC metastasis and the positive rate was 12.5%. Five cases with nasopharyngeal inflammation and neck lymphatic node proliferation were demonstrated to be EBV DNA negative. Conclusion This technique is rapid, sensitive and specific, which is valuable clinically, especially for the diagnosis in patients with cryptic nasopharyngeal carcinoma and with neck metastasis but lack of clear original focus.