摘要
试验以郎酒酒糟为原料,采用乙醇热回流法提取总酚,对提取和检测方法进行了优化研究,并检测了总酚的抗氧化性。研究表明,福林酚法检测总酚的最佳反应条件为福林酚试剂和Na2CO3溶液各为3mL,反应温度为25℃,反应时间为1h。乙醇回流法提取总酚的最佳工艺条件为乙醇浓度100%vol、料液比1∶11、提取时间8h、提取温度75℃,此时总酚含量为29.36%,其还原能力为56.32%。
Using Langjiu Liquor vinasse as raw materials, the total phenols were extracted by ethanol thermal refluxing method. The extraction and detection method was optimized and the antioxidation of the total phenols was detected, The results showed that the optimum parameters of determining the total phenols obtained by Folin phenolic method were as followed： Folin phenolic reagents and Na2CO3 solution 3ml, respectively, the reaction temperature 25℃ and the reaction time lb. The optimum conditions of extracting total phenols were obtained by orthogonal test as followed： ethanol concentration 100%, the solid-liquid ratio 1：11, extraction time 8h and the extraction temperature 75℃. Under these conditions, the total phenols content reach 29.36% and its reducing capacity reached 56.32%.
出处
《中国酿造》
CAS
北大核心
2011年第7期137-140,共4页
China Brewing
关键词
酒糟
总酚
提取
优化
还原能力
vinasse
total phenols
extraction
optimization
reducing capacity
