鸭PPARα基因结构及功能的生物信息学分析

Bioinformatics analysis of duck PPARα gene structure and function

以鸭的过氧化物酶体增殖物激活受体α(PPARα)基因为研究对象,运用生物信息学方法对其编码的蛋白质结构、理化性质及功能结构域进行分析.结果表明:鸭的PPARα基因cDNA全长1 430 bp,最长开放阅读框为1 407 bp,编码468个氨基酸;序列比对结果显示鸭的PPARα基因与鸡、胸草雀、人、牛、家马等该基因的核苷酸序列同源性分别为96%、92%、79%、77%和78%,鸭PPARα基因编码的氨基酸序列与上述其他动物的同源性高达98%、97%、89%、89%和88%;系统进化分析结果显示,在鸡、胸草雀、人、牛、马等动物中,鸭的PPARα基因与鸡的进化关系最为密切,亲缘关系最近;鸭的PPARα蛋白中α螺旋较多,该蛋白质含有1段短的核定位信号序列KKNRNKC,在引导该蛋白质进入细胞核的过程中发挥作用,同时还有由2个锌指结构组成的DNA结合域及1段配体结合域.

Protein structures,physical and chemical properties,and their functional domain structures encoded by duck peroxisome proliferators-activated receptors-α（PPARα） gene were analyzed using bioinformatics method.Sequencing and bioinformatics analysis showed that the duck PPARα cDNA sequence was 1430 base pairs long with an open reading frame（ORF） containing 1407 nucleotides encoding a protein of 468 amino acids.The multiple sequence alignment showed that coding nucleotide sequences of the obtained duck PPARα gene had 96%,92%,79%,77%,78% homology with the corresponding chicken,Taeniopygia guttata,human,bovine and Equus caballus,respectively,and the deduced amino acid sequences had 98%,97%,89%,89%,88% identity with the corresponding species,respectively.Phylogenetic tree analysis of the amino acid sequences of duck PPARα and other PPARα proteins in human and other animals showed that,among all the species mentioned above,the nearest relationship existed between duck and chicken.Further analysis on the structure and function of the duck PPARα protein showed that there were much more α helix than other secondary structure in the duck PPARα protein,besides,it had a short nuclear localization signal（KKNRNKC）,so that it could enter into nucleus.Moreover,a DNA binding domain which was composed of two C4-type zinc fingers and a ligand binding domain was found in the PPARα protein.