利用分子标记辅助选育油菜隐性上位互作核不育临保系

Marker-assisted selection for temporary maintainers of a recessive epistemic genic male sterility in Brassica napus L

以湘油15、沪油15、优88和沪油17分别与临保系HYZ1-TAM杂交,在其F2利用与不育基因Bnms3和隐性上位基因Bnrf连锁的分子标记对临保系基因型单株进行筛选,并对筛选获得的单株进行测交验证确定是否为临保系基因型.结果表明:在4个品种的转育F2群体共355个单株中,利用分子标记辅助选择获得了62个标记基因型为临保系的单株,这些单株经测交证实有57株为真实临保系,分子标记辅助选择的准确率为91.94%.根据实验结果推测,本研究所用的4个甘蓝型油菜品种在Bnms4基因位点未出现显性可育等位基因.实验结果验证了分子标记辅助选择具有较高的准确率,对开展该不育系统的转育工作具有很好的借鉴意义.

Four varieties of Brassica napus,Xiangyou 15,Huyou 15,You 88 and Huyou 17,were crossed with a temporary maintainer（TAM） named HYZ1-TAM respectively and produced four F2 populations.In these populations,molecular markers associated with Bnms3 and Bnrf were used to select new temporary maintainer plants which were identified by test-cross.Sixty-two plants with TAM marker-genotype were selected by marker-assisted selection（MAS） from three hundred and fifty-five plants of four F2 populations.Fifty-seven plants（with the accuracy of 91.94%） were TAM genotype proved by test-cross.Compared with traditional method,it is very efficient to select TAM by MAS.In the Bnms4 locus,all the four varieties have a same genotype which is ms4ms4.