摘要
文中设计了基于SPCE061A的PCR芯片温度控制系统。系统以集成在PCR芯片底面的铂薄膜电阻作为温度传感器和加热器;加热器采用双螺旋型结构,以提高PCR反应温度均匀性。在实时测量、PID控制中,为了提高测量精度,使用0.5 mA恒流源电路将温度信号转化为电压信号。实验结果表明:该系统的温度控制精度达到±1℃,升温速度达到13℃/s,降温速度达到6℃/s,能够满足快速PCR反应要求。
This paper introduced a design of PCR chip temperature control system based on SPCE061A.On the button of the PCR chip,Pt thin-film resistors were used as the temperature sensor and heater.To improve the temperature homogenization of PCR chip,the paper designed a double helix structure heater.In order to improve the measurement precision in real-time measurement and PID control,the system used a 0.5 mA constant-current circuit so as to transform temperature signal into voltage signal.Experimental results show that the precision of the temperature system reaches to ±1 ℃,and the rate of temperature increase reaches to 13 ℃/s while the cooling speed was 6 ℃/s,which can meet the requirements of rapid polymerase chain reaction.
出处
《仪表技术与传感器》
CSCD
北大核心
2011年第6期84-86,共3页
Instrument Technique and Sensor
基金
湖北省教育厅科学技术研究计划重点项目(D20101903)
