乳饮料中V_B_6的液相色谱检测方法

Dairy Products VitaminB_6 Liquid Chromatography Detection Methods

建立一种测定乳饮料中VB3含量的高效液相色谱法。样品经乙晴萃取后进样，采用C18250mm×4．6mm色谱柱分离，流动相为甲醇，三乙胺，辛烷磺酸钠。荧光检测；A-R261nm，RSD为0．56％-0．82％（n=5），标准曲线r=-0．9998，平均回收率分别为98．2％。

To establish a milk drink determination of VitaminB6 by HPLC method. The sample was extracted with acetonitrile, Cts 250 mm ×4.6 mm column with mobile phase of methanol, Triethylamine, Octane sulfonate, Fluorescent detection; A-R261 nm, RSD is 0.56 % -0.82 % （n=5）, the correlation coefficients were more than 0.999 8, the recoveries were 98.2 %.