灵芝蒜颗粒对小鼠急性酒精性肝损伤保护作用的研究

Protective Effect of Lingzhisuan Granulas on Acute Alcoholic Liver Injury of Mice

目的:研究灵芝蒜颗粒(LZS)对小鼠急性酒精性肝损伤的保护作用。方法:将60只昆明种小鼠随机分为空白组、模型组、LZS(高、中、低剂量)组和联苯双酯组,给药15 d,空白组和模型组小鼠灌胃给予生理盐水,其余各组一次性灌胃给予50%乙醇12 mL/kg,处死小鼠采集血液样品和肝组织样品,检测血清转氨酶、甘油三酯含量及肝组织丙二醛(MDA)、还原型谷胱甘肽(GSH)含量,并进行病理组织学观察。结果:与模型组比较,灵芝蒜各剂量组GSH含量降低(P<0.01),其中高剂量组ALT、AST、TG、MDA含量显著降低(P<0.05,P<0.01);病理切片表明灵芝蒜颗粒各剂量组肝组织损伤有不同程度减轻。结论:灵芝蒜颗粒对小鼠急性酒精性肝损伤有良好的治疗和保护作用。

Objective ：To study protective effect of Lingzhisuan（LZS）granulas on mice with acute alcoholic liver injury. Meth- ods：Sixty healthy KM mice were randomly divided into six groups：blank control group, model group, Lingzhisuan granulas high dosage group,middle dosage group,low dosage group and bifendate group. Mice in treatment groups were adminis- tered for 15 days,while mice in normal group and model group were given saline. After mice in treatment groups and mod- el group were given 50% alcohol at a dose of 12 mL/kg,they were sacrificed,and their blood samples and liver tissue samples were collected. At last,the levels of serum ALT,AST,TG and the levels of liver tissue MDA, GSH were deter- mined respectively, changes of pathohisology of mice were observed. Result：Compared with model group, GSH content was significantly declined in LZS treatment groups （P〈0.01）,contents of ALT,AST,TG,MDA in high dosage LZS group were significantly declined （P〈0.05 ,P〈0.01 ）.The pathological sections showed that LZS could reduced liver injury to some ex- tent. Conclusion ： Lingzhisuan granulas has protective and curative effects on acute alcoholic liver injury of mice.