不同灭菌方法对人参健脾丸含量的影响

Effect of Different Sterilization Methods on Costunolide Content in Renshen Jianpi Pills

目的:研究不同灭菌方法对人参健脾丸中木香烃内酯含量的影响。方法:采用高效液相色谱法对人参健脾丸中木香烃内酯进行含量测定,色谱柱:Kromasil C18;流动相:甲醇-水(70∶30);流速:1.0 mL/min;紫外检测波长:225 nm。结果:样品T1中木香烃内酯含量最高,样品T3中木香烃内酯含量最低。结论:对人参健脾丸应选用60Co辐照灭菌。

Objective：To research effect of different steriliration methods on costunolide content in Renshen Jianpi pills. Method：HPLC was performed on Kromasil C18 column with methanol-water （70：30）as mobile phase. The flow rate was at 1.0 mL/min and detection wavelength was set at 225 nm. Result：Costunolide content was highest in sample T1,but it was lowest in sample T3. Conclusion：Renshen Jianpi pills should be sterilized by 60Co irradiation.