苯丙氨酸与对苯醌荷移反应的研究

Study on Charge Transfer Reaction of Phenylalanine and Benzoquinone

目的：采用分光光度法研究苯丙氨酸与对苯醌的荷移反应。方法：将苯丙氨酸与对苯醌在pH=8．5的三酸缓冲溶液中，90℃水浴加热30min的条件下发生反应，并对生成物进行分光光度法研究。结果：荷移络合物的hmax=339nm，表观摩尔吸光系数ε=2．4×10^3L／（moL·cm），在20μg／mL～60μg／mL的范围内符合比尔定律，回收率在99．7％～103．0％范围内，相对标准偏差在2．57％以内。结论：应用此方法测定苯丙氨酸样品含量与使用药典法测量结果一致。

Objective:Charge transfer reaction between phenylalanine and benzoquinone was studied by spectrophotometry.Methods:Phenylalanine was reacted with benzoquinone in buffer solution(pH 8.5)at 90 ℃ water bath for 30 min,and complex was studied by spectrophotometry.Results:Apparent molar absorptivity was 2.4×103 L/(moL·cm)at 339 nm.Beer′s Law was obeyed in the range of 20 μg/mL-60 μg/mL,the recovery was 99.7%~103.0% and relative standard deviation was less than 2.57%.Conclusion:Experimental results are in good agreement with those obtained by official method.