里氏木霉Trichoderma reesei产纤维素酶的发酵培养基碳氮源优化

Culture medium optimization for producing cellulase by Trichoderma reesei

研究C、N源对里氏木霉(Trichoderma reesei)生产纤维素酶的影响,采用单因素实验方法和中心复合方法对发酵培养基进行优化。单因素实验表明:黄豆饼粉、玉米芯、玉米浆对纤维素酶的影响显著。通过响应面优化,得到最优培养基C、N源的组成:黄豆饼粉32.21 g/L,玉米芯42.29 g/L,玉米浆4.45 g/L。优化条件下,摇瓶发酵7 d的比酶活达到(10.65±0.50)U/mL。

The influence of carbon and nitrogen sources on cellulase production was investigated. Single factor method and response surface methodology were used to optimize the medium for cellulase production by Trichoderma reesei. Firstly, it showed that soybean powder, corn cob and corn steep liquor had positive effects on cellulase production in one factor at a time. Then, the optimal concentrations of soybean powder （32. 21 g/L）, corn cob （42. 29 g/L） and corn steep liquor （4. 45 g/L） were determined by central compostion design cellulase production reached and response surface analysis. Under these optimizations conditions, （10.65 ±0. 50） U/mL on the flask cultivation for 7 d.