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酶的定向进化研究及其在工业生物催化中的应用 被引量：8

Recent advances in directed evolution of enzymes and its application in industrial biocatalysis
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摘要 综合概括了各种蛋白质改造与微生物代谢途径改造的非理性定向进化技术的原理、特点,介绍了这些技术在高性能工业生物催化剂改造中的应用。 This review summarized the principles, characteristics, progress and the research direction of protein and metabolic pathway. Successful samples in high performance industrial bioeatalyst were presented.
作者 苏龙 庄宇 何冰芳
机构地区 南京工业大学生物与制药工程学院 玉林师范学院生命科学与技术学院
出处 《生物加工过程》 CAS CSCD 2011年第4期69-75,共7页 Chinese Journal of Bioprocess Engineering
基金 国家重点基础研究发展计划(973计划)资助项目(2009CB724700) 国家高技术研究发展计划(863计划)资助项目(2007AA021306)
关键词 定向进化 DNA改组 易错PCR 工业生物催化 directed evolution DNA shuffling error-prone PCR industrial biocatalysis
分类号 Q814 [生物学—生物工程] Q819 [生物学—生物工程]
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参考文献42

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二级参考文献64

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生物加工过程
2011年 第4期

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