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HPLC法测定伸筋片中士的宁与马钱子碱的含量 被引量:1

Determination of strychnine and brucine in Shenjin Pill by HPLC
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摘要 目的采用高效液相色谱法测定伸筋片中士的宁与马钱子碱的含量。方法 C18色谱柱,流动相为乙腈-0.01 mol.L-1庚烷磺酸钠与0.02 mol.L-1磷酸二氢钾等量混合溶液(用10%磷酸调节pH值2.8)(21∶79),检测波长为260 nm。结果士的宁进样量在0.064~0.320μg范围内呈良好的线性关系(r=0.998 8),平均加样回收率99.31%,RSD=0.57%(n=6),马钱子碱进样量在0.056~0.280μg范围内呈良好的线性关系(r=0.997 3),平均加样回收率99.35%,RSD=0.42%(n=6)。结论本方法简便、准确、重复性好,为建立样品含量限度提供依据。 Objective To establish an HPLC method for the determination of strychnine and brucine in Shenjin Pill.Methods C18 column was used.The mobile phase was acetonitrile-0.01 mol·L-1 sodium heptanesulfonate mixed with same quantity of 0.02 mol·L-1 potassium dihydrogen phosphate(adjusted pH 2.8 by 10% phosphoric acid)(21∶79),and the detection wavelength was 260 nm.Results The linear range of strychnine was 0.064~0.320 μg(r=0.998 8),the average recovery was 99.31%,and the RSD was 0.57%(n=6).The linear range of brucine was 0.056~0.280 μg(r=0.997 3),the average recovery was 99.35%,and the RSD was 0.42%(n=6).Conclusion The method was simple,accurate with good reproducibility and provided a reliable basis for establishing the content limitation of samples.
出处 《齐鲁药事》 2011年第7期389-391,共3页 qilu pharmaceutical affairs
关键词 伸筋片 士的宁 马钱子碱 高效液相色谱法 Shenjin Pill Strychnine Brucine HPLC
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