沙棘叶水提物适应原性及安全性评价

Adaptogenic and Safety Evaluation of Seabuckthorn(Hippophae rhamnoides) Leaf Extract a dose Dependent Study

本文评估了沙棘叶水提物在大白鼠体内的适应原活性和毒性。提取物的剂量依赖性适应原活性的研究采取将小鼠置于低温(5℃),低压(428mmHg),抑制(C-H-R)的环境下的30min前口服不同剂量的沙棘叶水提物。在亚急性毒性中,连续14d每天口服10倍和20倍最大有效剂量(每天口服1g/kg体重和2g/kg体重)和连续30d每天口服最大有效剂量(每天口服100mg/kg体重)后,研究血清和血液的生化参数和血液参数的变化。发现相对于对照组,重要器官的器官重量/体重比无显著变化(除肝脏和肾脏在1g/kg体重剂量和2g/kg体重剂量)。实验组小鼠的生化参数和血液参数也无显著变化。在急性毒性试验中,口服沙棘叶水提物时,提取物的LD50大于10g/kg体重。这些结果表明,沙棘叶水提物在亚急性(30d)下给予最大有效剂量也具有潜在的无毒的适应原活性。

The effects of seabuckthorn（Hippophae rhamnoides L.,Elaeagnaceae）,leaf aqueous extract were examined in rats for its adaptogenic activity and toxicity.Dose dependent adaptogenic study of extract was carried out at different doses administered orally,30 minprior to cold（5℃）-hypoxia（428mmHg）-restraint（C-H-R） exposure.After sub-acute toxicity studies on 10 and 20 times doses of maximal effective dose administered for 14 days（single oral dose of 1g/kg and 2g/kg once daily） and maximal effective dose administered for 30days（single oral dose of 100mg/kg once daily）,biochemical and hematological parameters were studied in the serum and blood.The maximal effective adaptogenic dose of the extract was 100mg/kg body weight.No signi cant changes were observed in organ weight/body weight ratios,of any vital organ studied（except liver and kidney in 1g/kg and 2g/kg body weight doses,r espectively）,And biochemical and hematological parameters of the sub-acute drug treated animals incomparison to control rats.In acute toxicity Study LD50 of the extract was observed to be10g/kg when given orally.These results indicate that seabuckthorn leaf aqueous extract Possess potent adaptogenic activity with no toxicity even after sub-acute（30 days） maximal effective dose administration.