摘要
目的:采用大鼠心房纤维化的模型,探讨转化生长因子-β1(TGF-β1)对大鼠心房纤维化和缝隙连接蛋白(Cx)40重构的影响及阿托伐他汀的防治作用。方法:将30只雄性Wistar大鼠随机等分为对照组(A)、盐酸异丙肾上腺素组(B)、盐酸异丙肾上腺素+阿托伐他汀组(C)。8周后处死,Masson染色法测大鼠心房纤维化程度,免疫组化法显示大鼠心肌组织TGF-β1和Cx40分布特征,并半定量统计分析。rt-PCR测大鼠心肌组织中Cx40mRNA表达情况。结果:与A组相比,B组大鼠心肌组织TGF-β1含量显著升高(P<0.01),心房纤维化程度增加,Cx40含量显著减少(P<0.01),且分布紊乱;与B组相比,C组大鼠心肌组织中TGF-1β含量减少(P<0.05),心房纤维化程度明显减轻,Cx40含量升高(P<0.05),分布基本恢复正常;与A组相比,C组大鼠各指标均无明显变化(P>0.05)。结论:心肌组织中TGF-β1含量增加可能是心房纤维化和Cx40重构的机制之一;阿托伐他汀可通过降低心肌组织中TGF-β1含量显著改善心房纤维化程度和Cx40重构。
Objective： To observe the effects of TGF-β1 on atrial myocardial fibrosis and connexin40 remodeling in rats and the protection of atorvastatin. Methods： 30 male Wistar rats were randomly divided into three groups. 8 weeks after the first intragastric administration, all the survival rats were anesthetized. The degree of atrial fibrosis was measured with Masson staining. Immunohistochemistry was used to measure the level and the distribution of TGF-β1 and Cx40. RT-PCR was used to measure the expression of Cx40 mRNA. Results：Compared with group A, the level of TGF-β1 and the degree of atrial fibrosis in the group B were markedly increased （P〈0.01）, while the mRNA expression and the level of Cx40 were decreased and the distribution was confused. Compared with group B, the level of TGF-β1 and the degree of atrial fibrosis in the group C were reduced （〈0.05）, while the mRNA expression and the level of Cx40 were elevated and the degree of confusion was reduced. Compared with group A, all the points of group C were unchanged. Conclusion：The increase of TGF-β1 in myocardium may be one of the important mechanism of atrial fibrosis and Cx40 remodeling, which can be inhibited effectively by atorvastatin.
出处
《国际心血管病杂志》
2011年第4期234-237,共4页
International Journal of Cardiovascular Disease
