应激状态下大鼠胃粘膜疏水性及表面活性磷脂的变化

THE CHRONOLOGICAL CHANGES IN GASTRIC MUCOSAL HYDROPHOBICITY AND THE CONTENT OF SURFACE ACTIVE PHOSPHOLIPID IN RATS UNDER WATER IMMERSION STRESS CONDITIONS

为探讨应激对胃粘膜疏水性及胃粘膜表面活性磷脂的影响，采用大鼠水浸束缚方法制作应激模型，检测应激前及应激后０．５、１ 及２ｈ 时大鼠腺胃粘膜接触角、粘液凝胶层磷脂含量及胃粘膜病变程度。结果发现，各组大鼠腺胃粘膜接触角分别为（３８．０±２．４）°、（２６．８±１．８）°、（２０．０±１．６）°和（１４．４±３．３）°；每克腺胃组织磷脂含量分别为（４６．７６±４．７３）μｇ、（３２．４９±９．４５）μｇ、（２５．５１±５．７８）μｇ 和（１８．１４±７．９１）μｇ；应激后腺胃区胃粘膜损伤指数积分分别为０、２．８±１．０、６．０±１．６和１１．４±３．１。与应激前比较，应激后胃粘膜接触角、磷脂含量及粘膜病变程度差异有显著性意义（Ｐ＜ ０．０１），且胃粘膜接触角与磷脂含量呈明显相关性（ｒ＝ ０．７６），两者与胃粘膜病变程度呈显著的负相关（ｒ＝ － ０．９５）。结果提示，应激状态下胃粘膜接触角与粘液凝胶层磷脂含量进行性降低，胃粘膜病变程度逐渐加重，提示胃粘膜疏水性的降低可能参与了应激性溃疡的发生，其降低的机制与粘液凝胶层磷脂含量降低有关。

The aim of the study was to analyze the impact of stress on gastric mucosal hydrophobicity and the dontent of phospholipid in gastric glandular adherent mucus gel layer in rat. Forty rats were randomly divided into four groups, which were control group, 0.5, 1 and 2 h after water immersion restraint stress groups. The gastric mucosal contact angle and the content of phospholipid in mucosal surface scraping material (SSM) of glandualar stomach and gastric mucosal ulcer index were measured. The gastric mucosal contact angle in each group was (38.0±2.4)°、(26 8±1 8)°、(20 0±1 4)° and (14 4±3 3)° respectively. The content of phospholipid in SSM per gram of glandular stomach in each group were (46 76±4 73)、(32 49±9 45)、(25 51±5 78)、(18 14±7 91)、(36 76±4 73)μg respectively and the ulcer index was 0、2.8±1.0、6.0±1.6 and 11.4±3.1 respectively. The three parameters showed significant changes in stress conditions as compared with those in normal control ( P <0.01). the level of contact angle and the content of phospholipid in mucosal SSM were observed to decrease proportionally after stress and both of them were negatively correlated with ulcer index(r=-0.95). It suggests that the decrease in gastric mucosal hydrophobicity, which possibly results from a decrease in the content of phospholipid in mucus gel layer, may be one of the causative factors of stress ulcer.