红细胞在一氧化氮储存和运输中的作用

THE ROLE OF ERYTHROCYTE IN STORAGE AND TRANSPORT OF NITRIC OXIDE

通过定量比较红细胞内外一氧化氮（ＮＯ）浓度，观察ＮＯ产生体系对红细胞内ＮＯ 浓度变化的影响，外源性ＮＯ对氧合血红蛋白、脱氧血红蛋白的影响以及ＮＯ对硫醇类物质影响，探讨红细胞在ＮＯ储存和运输过程中的作用。结果发现红细胞内的ＮＯ浓度显著高于血浆，加入ＮＯ产生体系，红细胞中ＮＯ浓度增加；外源性ＮＯ可以结合到脱氧血红蛋白的铁离子上，并使氧合血红蛋白向高铁血红蛋白转换；ＮＯ 可结合到硫醇类物质（Ｌ－半胱氨酸）的巯基上形成亚硝基硫醇。这些结果表明：ＮＯ进入红细胞内可避免Ｏ２ 的破坏而被安全地储运。

The aim of the study was to elucidate the role of erythrocytes on the storage and transport of NO. the NO concentration in erythrocytes and in plasma were quantitated, the effect of NO producing system on NO level in erythrocytes was analyzed, and the impact of oxogenous NO on oxyHb,deoxyHb and thiol (L cysteine) was studied. The results showed that NO concentration in erythrocyte was much higher than that in plasma, that the NO level was increased in erythrocyte with the addition of NO producing system, that NO could bind to deoxyHb and convert oxyHb into methemoglobin very rapidly, and that NO reacted with thiol to form s nitrosothiol. These results confirmed that NO in erythrocyte could avoid its destruction by O 2 so that in could be stored and transported safely. The transport of NO by the erythrocyte might be controlled by the allosteric effect of hemoglobin structure.