全身冲击伤后脑组织内NO变化及其与神经行为功能的关系

CHANGES IN NO IN BRAIN TISSUES AND ITS RELATIONSHIP WITH NEUROBEHAVIORAL FUNCTION AFTER WHOLE BODY BLAST INJURY

观察了全身冲击伤（ＷＢＢＩ）后大鼠海马和脑干组织内亚硝酸盐／硝酸盐（ＮＯ－２ ／ＮＯ－３ ）含量变化及其与神经行为功能改变的关系。结果表明：①ＷＢＢＩ后３、２４ 和１２０ｈ，海马和脑干组织内ＮＯ－２ ／ＮＯ－３ 、ｃＧＭＰ含量明显升高，与冲击伤伤情呈显著正相关；②ＷＢＢＩ后，大鼠主动回避反应（ＡＡＲ）明显下降，主动回避反应时间（ＡＡＲＬ）、逃避反应时间（ＥＲＬ）明显延长；③ＷＢＢＩ后，海马和脑干组织内ＮＯ－２ ／ＮＯ－３ 水平分别与ＡＡＲ、ＥＲＬ和ＡＡＲＬ值呈显著相关性。研究结果提示：ＷＢＢＩ时海马和脑干组织内ＮＯ含量明显增加，过量的ＮＯ释放与冲击伤致中枢神经系统功能性损害有一定关系。

This study was to investigate the changes in NO - 2/NO - 3 content in the hippocampus and brainstem and its relationship with neurobehavioral alteration after whole body blast injury (WBBI) in rats. It was found that the contents of NO - 2/NO - 3 and cGMP in the hippocampus and brainstem were significantly increased 3, 24 and 120h after WBBI, and they were markedly correlated with blast injury severity. Activeavoidance response (AAR) decreased obviously, and active avoidance response latency (AARL) and escape response latency (ERL) were significantly prolonged after WBBI. The correlation analysis indicated that the changes in NO - 2/NO - 3 in brain tissues were also significantly correlated with AAR, ERL and AARL respectively. The results suggested that NO production was significantly increased in the hippocampus and brainstem after whole body blast injury. The over release of NO might be related to the blast wave induced damage to the central nervous system.