丹参抗LDL的脂质过氧化和脑缺血损伤的研究

THE EFFECT OF RADIX SALVIAE MILTORRHIZAE (RSM) ON LIPOPEROXIDATION OF LDL AND ISCHEMIC BRAIN INJURY IN CHOLESTEROL FED RABBITS

给新西兰大白兔分别喂１％ 胆固醇食（ｎ＝ １９）和标准食（ｎ＝ １５）共５ 周，测定了血脂和低密度脂蛋白（ＬＤＬ）中的脂质过氧化物（ＬＰＯ）含量，并制成氧化型ＬＤＬ（ｏｘＬＤＬ），提取ｏｘＬＤＬ中的脂质进行薄层层析，并制成脑缺血模型，观察丹参抗脑缺血损伤的作用。结果：胆固醇食组兔血清胆固醇（ＣＨ）、磷脂（ＰＬ）、游离脂肪酸（ＦＦＡ）以及血浆和ＬＤＬ中ＬＰＯ 含量均显著高于标准食组（Ｐ＜ ０．０１）；胆固醇食兔ＬＤＬ和Ｃｕ２＋ 透析后的ｏｘＬＤＬ中的脂质成分发生变化，Ｘ 带增加，ＦＦＡ 带增高，而胆固醇酯（ＣＥ）带及ＴＣ带显著减少，而且丹参透析组脂质成分变化不显著，丹参组缺血后海马超微结构损伤较盐水组轻。表明，长期高胆固醇饮食可引起较多的ＬＰＯ和ｏｘＬＤＬ生成，从而加速动脉粥样硬化的形成；Ｘ 带可作为脂质过氧化的一个指标，丹参具有拮抗ＬＤＬ 氧化的作用，这是其对抗脑缺血损伤的机制之一。

The blood lipids and lipid peroxide (LPO) were analysed in rabbits fed with 1% cholesterol in feeding ( n =19) and with standard feeding ( n =15) after 5 weeks of feeding.The plasma LDL was isolated and ox LDL was obtained by thin layer chromatography (TLC).Cerebral ischemia was induced by ligation of left common carotid artery.The antioxidation and antiischemia injury property of RSM was also observed with TLC and electron microscopy.The results showed that the plasma LPO,FFA,CH and PL levels in 1% cholesterol fed rabbits were much higher than those of rabbits with standard feeding( P <0 01).The lipid constituent changes in LDL in 1% cholesterolfed rabbits showed that the spot X was higher than that of standard feeding group.After dialysis with Cu 2+ ,the spot X and FFA were increased,but CE and TG bands were decreased,and the percentage of spot X in RSM dialysed was significantly lower than that of Cu 2+ dialysed LDL.The ultrastructural changes in hippocampus were more severe in saline treated group than those of RSM treated group.The results indicated that high cholesterol intake may induce disturbances of lipid metabolism and increase the formation of LPO,the spot X may be an important indicator for lipoperoxidation. RSM plays a potential role in antioxidartion of LDL and in the prevention of brain injury brain during cerebral ischemia.