丙酮酸盐对败血症大鼠存活率的影响

The Influence of Pyruvate on the Survival Rate of Experimental Septicemia Rats

为研究丙酮酸盐对败血症大鼠晚期炎症介质HMGB-1血清浓度及存活率的影响。采用盲肠结扎穿孔方法建立败血症大鼠模型,应用随机数字表随机分为5组(假手术组,空白对照组,生理盐水组,阳性对照组,丙酮酸盐组)。运用酶联免疫吸附试验检测各组大鼠血清中TNF-αI、L-6及HMGB-1等炎症因子,比较浓度变化情况。Ka-plan-Meier法对各组大鼠存活率进行分析,比较不同剂量丙酮酸盐,不同时间点给药对败血症大鼠存活率的影响。结果显示:各组大鼠血清TNF-αI、L-6浓度在模型建立后即开始增高,空白对照及生理盐水组较其它3组增高明显(P<0.05),在建模后第2 d达最高值后开始下降。各组HMGB-1浓度在模型建立后第2 d开始显著增高,丙酮酸盐组及假手术组较其它3组增加缓慢(P<0.05),到第5 d达最高值后均开始下降。丙酮酸盐的最佳作用浓度为4mg/kg体重,最佳给药时间为模型建立后24 h。与其它四组相比,丙酮酸盐能显著降低败血症大鼠的血清HMGB-1浓度(P<0.05),对败血症大鼠存活率的影响,丙酮酸盐组与生理盐水组及空白对照组相比差异有统计学意义(P<0.05),与假手术组及阳性对照组相比差异无统计学意义(P>0.05)。由此可知,丙酮酸盐能显著降低败血症大鼠血清HMGB-1浓度,提高败血症大鼠的存活率,对败血症大鼠具有一定的治疗作用。

To investigate the influence of pyruvate on HMGB-1 concentration and survival of sepsis rats.The rats were executed cecal ligation and puncture for experimental sepsis which randomly divided into 5 groups（fake operation,blankcontrol,physiological saline,positive control and pyruvate）.The inflammation factors,such as TNF-α,IL-6 and HMGB-1 concentration were tested by ELISA.We compared the rates of different groups by Kaplan-Meier method.Then,we assess pyruvate these inflammation factors concentration in different group.To assess the survival effects on experimental septicemia rats at different dose-rates and in different administration time.After cecal ligation and puncture,TNF-αand IL-6 concentration of all groups obviously increased at once,after two days the concentration to reach the peak,and then began to decline.We compared the uptrend,blank control and physiological saline group was obviously higher than other three groups（P〈0.05）.The HMGB-1 concentration of all groups obviously increased after cecal ligation and puncture two days,the concentration to reach the peak after 5 days,and then began to decline.We compared the uptrend,fake operation and pyruvate group obviously slower than other three groups（P〈0.05）.The best concentration and administration time of pyruvate to experimental sepsis rats was 4mg per kilogram of body weight and 24 hours after executed cecal ligation and puncture respectively.Compared the other four groups,pyruvate significant decreased the HMGB-1 concentration of experimental sepsis rats.Compared the survival rate,the pyruvate group was obviously higher than and blank control and physiological saline group（P〈0.05）.Pyruvate can significant decreased the HMGB-1 concentration of experimental septicemia rats,also can improve the survival rate of experimental sepsis rats.Pyruvate has a certain therapeutic action for experimental septicemia rats.