siRNA沉默S100P基因对胰腺癌细胞的生长抑制作用

The Growth Inhibition Effect of S100P Gene Silencing by SiRNA on Pancreatic Cancer Cell Line SW1990

[目的]探讨小干扰RNA(siRNA)沉默S100P基因后对胰腺癌SW1990细胞株生长的影响。[方法]化学合成针对S100P基因的siRNA,用脂质体转染试剂将siRNA体外转染至人胰腺癌SW1990细胞中,48h后收集细胞,分别提取细胞总RNA和蛋白。用实时定量RT-PCR测定S100P基因mRNA水平的表达;免疫印迹测定S100P蛋白的表达;MTT法测定细胞的生长曲线。[结果]转染siRNA后,S100P基因在mRNA水平上表达减少了77%,蛋白表达降低了74%,均显著性低于对照组。siRNA-S100P明显抑制细胞生长。[结论]应用RNAi技术沉默S100P基因可以降低人胰腺癌SW1990细胞株中S100P表达,抑制细胞生长。

[Purposel To investigate the growth inhibition effect of SLOOP gene silencing by siRNA on human pancreatic cancer cell line SW1990. [Methods] The SLOOP gene silencing by siRNA was chemically synthesized and transfected into SW1990 cells. The cells were collected at 48h later, and the total RNA and protein was extracted. The mRNA and protein expression of SLOOP were detected by real-time quantitative PCR and Western blot. The cell growth curve was analyzed by MTT assay. [Resultsl After transfected with siRNA, SLOOP mRNA and protein of SW1990 cells were significantly decreased, with inhibition rate of 77% and 74% respectively. The cell growth was significantly inhibited. [Conclusion ] SLOOP gene silencing by siRNA can reduce the expression of SLOOP in pancreatic cancer cell line SW1990, and can inhibit the cell growth.