CyclinD1 mRNA反义寡脱氧核苷酸导入对颊癌细胞株BcaCD885生物学行为的影响

Effects of an Anti-Sense Oligodeoxynucleotides of CCND1 on the Biological Behaviors of BcaCD885

目的研究CyclinD1 mRNA反义寡脱氧核苷酸导入对颊癌细胞株BcaCD885生物学行为的影响,验证CyclinD1在口腔黏膜癌变中的作用。方法采用差示PCR技术检测颊癌细胞株BcaCD885中CyclinD1编码基因的扩增,并以脂质体为载体,将CyclinD1 mRNA的反义寡核苷酸序列转染肿瘤细胞,观察转染前后细胞增殖速度、体外集落形成能力的变化。结果 BcaCD885细胞株中出现CCND1基因扩增,扩增倍率6.9;转染CyclinD1mRNA的反义寡脱氧核苷酸后,细胞增殖速度减慢,体外集落形成能力下降。结论转染CyclinD1 mRNA反义寡脱氧核苷酸,能部分抑制体外培养肿瘤细胞的增殖活性和恶性表型。

Objective To investigate the roles of CyclinD1（CCND1） in oral carcinogenesis and then to explore the possibility of using an anti-sense oligodeoxynucleotides in an initial gene therapy trial.Methods The identical differential PCR was used to evaluate the amplification of CCND1 in human oral squamous cell carcinoma cell lines BcaCD885.Then,an anti-sense oligodeoxynucleotides complementary to human CCND1 mRNA was transferred using leptofectin as a vector into BcaCD885 cell line.The growth patterns of this cell line before and after the transferation were compared with each other,as well as with an ODN-free blank transferation.Results BcaCD885 demonstrated the amplification of CCND1,and decreased cell growth was noted in BcaCD885 cell line,treated with anti-sense ODN.Conclusion These results suggest that the amplification of CyclinD1（CCND1） may contribute to the development and progression of oral carcinogenesis.Anti-sense ODN of CCND1 may decrease excess cell proliferation of BcaCD885.Further studies need to be conducted to ascertain the role of anti-sense ODN of CCND1.