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肝癌细胞中与glypican3相关的信号转导分子表达分析 被引量：2

The Expression of Glypican3 and Related Signal Transduction Molecules in Hepatocellular Carcinoma
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摘要 [目的]探讨肝细胞性肝癌(hepatocellular carcinoma,HCC)中与硫酸乙酰肝素糖蛋白3(glypican3,GPC3)表达相关的Wnt信号通路及其他信号转导分子表达。[方法]通过实时荧光定量PCR方法检测GPC3过表达的肝癌细胞株以及不同肝病变组织样本中GPC3、Wnt、基质金属蛋白酶(MMPs)、内皮硫酸脂酶及其他信号分子表达。[结果]肝癌组织GPC3mRNA水平显着高于良性肝病变组织(P<0.05)。肝癌GPC3的表达与MMP2、14,内皮硫酸脂酶1、2,成纤维细胞生长因子及其受体FGF2、FGFR1,以及胰岛素样生长因子受体IGFR1R的表达具相关性。[结论]GPC3与MMPs、FGF和IGF结合,可能在肝癌的发展中起重要作用。 [Purpose] To investigate the association of glypican-3（GPC3） expression with Wnt and other signaling transduction molecules in hepatocellular carcinoma（HCC）.[Methods] Expression of GPC3,Wnt,matrix metallo proteinases （MMPs）,sulfatase1 （SULF1）,SULF2 and other signaling molecules in GPC3-transfected HCC cells and in various liver tissue samples was analyzed by real-time reverse transcription-polymerase chain reaction.[Results] GPC3 mRNA was significantly higher in patients with HCC that in non-HCC subjects（P0.05）.GPC3 expression was correlated with expressions of MMP2,MMP14,SULF1,SULF2,fibroblast growth factor receptor 1（FGFR1）,insulin-like growth factor1 receptor（IGF1R）.[Conclusion] GPC3,in conjunction with MMPs,FGF and IGF,might play an important role in the carcinogenesis of HCC.
作者 吴飞翔 黄山 马良 张志明 刘剑勇 袁卫平 赵荫农
机构地区 广西医科大学附属肿瘤医院
出处 《中国肿瘤》 CAS 2011年第7期521-524,共4页 China Cancer
关键词 硫酸乙酰肝素糖蛋白3 WNT信号 基质金属蛋白酶 硫酸脂酶 肝细胞肝癌 glypican-3 Wnt sigualing matrix metalloproteinases sulfatase hepatocellular carcinoma
分类号 R735.7 [医药卫生—肿瘤]
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参考文献14

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同被引文献26

  • 1Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ),Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ),Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG).Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma[J].Hepatobiliary & Pancreatic Diseases International,2011,10(3):289-294. 被引量：53
  • 2缪辉来,谢贵林,温继育,陈念平,陈明,包仕廷.Glypican-3蛋白在肝细胞癌患者血清和组织中的表达及意义[J].世界华人消化杂志,2007,15(21):2311-2315. 被引量：13
  • 3Timek DT,Shi J,Liu H. Arginase-1,HepPar-1,and glypican-3 are the most effective panel of markers in dis-tinguishing hepatocellular carcinoma from metastatic tumor on fine-needle aspiration specimens[J].{H}American Journal of Clinical Pathology,2012,(2):203-210.
  • 4Zhang L,Liu H,Sun L. Glypican-3 as a potential differential diagnosis marker for hepatocellular carcino-ma:a tissue microarray-based study[J].{H}ACTA HISTOCHEMICA,2012,(6):547-552.
  • 5Dedes PG,Gialeli Ch,Tsonis AI. Expression of ma-trix macromolecules and functional properties of breast Cancer cells are modulated by the bisphosphonate zole-dronic acid[J].{H}Biochimica et Biophysica Acta,2012,(12):1926-1939.
  • 6Fjeldstad K,Kolset SO. Decreasing the metastatic poten-tial in cancers--targeting the heparan sulfate proteoglycans[J].{H}Current Drug Targets,2005,(6):665-682.
  • 7Li L,Jin R,Zhang X. Oncogenic activation of glypi-can-3 by c-M yc in human hepatocellular carcinoma[J].{H}HEPATOLOGY,2012,(4):1380-1390.
  • 8Zarrabi K,Dufour A,Li J. Inhibition of matrix met-alloproteinase 14(MMP-14)-mediated Cancer cell migra-tion[J].{H}Journal of Biological Chemistry,2011,(38):33167-33177.
  • 9Koyama Y,Naruo H,Yoshitomi Y. Matrix metallo-proteinase-9 associated with heparan sulphate chains of GPI-anchored cell surface proteoglycans mediates motility of murine colon adenocarcinoma cells[J].{H}Journal of Biochemistry,2008,(5):581-592.
  • 10Hav M,De Potter A,Ferdinande L. Glypican-3 is a marker for solid pseudopapillary neoplasm of the pancreas[J].{H}HISTOPATHOLOGY,2011,(6):1278-1279.

引证文献2

二级引证文献25

中国肿瘤
2011年 第7期

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