摘要
目的:建立灯盏花素片溶出度测定的方法。方法:以磷酸盐缓冲液(pH 6.8)为溶出介质,按《中国药典》2010年版二部附录XC第二法操作,用紫外分光光度法检测,计算灯盏花素片的溶出度。结果:灯盏花素在5.30～12.38μg·ml^(-1)范围内浓度和吸光度成良好线性,r=0.999 9。本法平均回收率为97.25%,RSD为0.94%。溶出度受转速影响小,受溶剂影响大。结论:本方法简单、准确、重复性好,可用于灯盏花素片的质量控制。
To establish a method for determing the dissolution of Breviscapinun tablets. Method： Breviscapin release was investigated in phosphate BS（ pH6. 8 ） by the second method for the release determination （ Chinese Pharmacopoeia 2010 edition）. Breviscapin content was assayed by UV method and the release percentage was then calculated. Result： Good linear relationship was shown in the concentration range of 5.30-12. 38 μg·ml -l （ r = 0. 999 9 ）. The average recovery of scutellarin was 97. 25% （ RSD = 0. 94% ） o The dissolution was almost unaffected by rotation rate,but greatly affected by the type of solvent. Conclusion： The method is simple, accurate, rapid and reproducible, and suitable for quality control of Breviscapinun tablets.
出处
《中国药师》
CAS
2011年第7期1014-1016,共3页
China Pharmacist
关键词
灯盏花素片
野黄芩苷
溶出度
紫外分光光度法
Breviscapinun tablets
Scutellarin
Release
Uhravioletvisible absorption spectroscopy
