恶性疟原虫保护性抗原复合基因—痘苗病毒重组活疫苗株家兔免疫血清及IgG对恶性疟原虫的体外抑制试验

INHIBITION OF RABBIT SERA AND IgG TO PLASMODIUM FALCIPARUM INDUCED WITH A VACCINIA VECTORED MULTI EPITOPE LIVE VACCINE CANDIDATE

用恶性疟原虫痘苗病毒重组活疫苗候选株的家兔免疫血清及纯化的ＩｇＧ 进行了恶性疟原虫的体外培养抑制试验，并与该目的基因的原核表达蛋白及全虫抗原进行了比较。结果在疟原虫的第一个生长周期内，重组痘苗病毒组的免疫血清抑虫作用最强（Ｐ＜ ０．０５），其次为全虫抗原及原核蛋白组；ＩｇＧ 则在第一及第二个生长周期均以原核表达蛋白组抑虫作用最强（ Ｐ＜ ０．０５） ，其次为重组痘苗病毒及全虫抗原组。说明重组痘苗病毒免疫血清及ＩｇＧ具有一定的体外抑制恶性疟原虫增殖作用，值得进一步深入研究。

Rabbit sera and IgG induced with a vaccina virus vectored multi epitope live vaccine candidate were tested for inhibition to the multiplication of Plasmodium falciparum(P.f.) in vitro. The prokaryotic expression protein of this multi epitope gene and whole parasite antigen were also compared. Results showed that the group of anti recombinant vaccinia virus sera had a strongest capability of inhibiting P.f. in 24 hours(first cycle), and the groups of prokaryotic expression protein and whole parasite antigen next. In IgG experiments, the group of prokaryotic expression protein showed a strongest inhibiting ability, the next were the groups of recombinant vaccinia virus and whole parasite antigen in 24 and 72 hours(first and second cycle). These indicated that the rabbit anti recombinant vaccinia virus live vaccine candidate had some inhibition ability to the multiplication of P.f. in vitro .