摘要
对360份山葡萄种质资源进行了遗传多样性分析研究。从245对引物中筛选出18对用于供试材料的SSR扩增；单条引物扩增条带为4～13条，平均9．44条；扩增产物长度介于150～1000bp，以200-750bp的扩增片段居多；18对引物共扩增出170条带，其中多态性条带167条，多态性百分率为98．2％；Shannon’s多样性指数（I）为1．778051。某些品种（系）产生了特有SSR标记带，共5条，占2．95%。
The analysis of genetic diversity of 360 Vitis arnurensis Rupr. germplasm resources was conducted in the paper. 18 pairs of primers were selected from 245 primers for SSR amplification. A single primer amplified 4-13 bands,average 9.44. Amplified product length was between 150-1 000 bp,and the fragments of 200-750 bp were majority. 18 pairs of primers amplified 170 bands totally, and 167 bands were polymorphic. The polymorphic percentage was 98.2 % and the Shannon＇ s diversity index（I）was 1. 778051. Some materials produced some unique bands in 360 Vitis amurensis Rupr. germplasm resources. A total of five unique bands were detected, accounting for 2.95%
出处
《北方园艺》
CAS
北大核心
2011年第14期102-104,共3页
Northern Horticulture
基金
现代农业产业技术体系建设专项资金资助项目(nycytx-30)
关键词
山葡萄
SSR
遗传多样性
Vitis amurensis Rupr.
SSR
genetic diversity
