分蘖洋葱叶片RNA提取方法的比较和分析

Comparison of Different Methods for Total RNA Extraction from Leaves of Tillering Onions

以新鲜的分蘖洋葱叶片为材料，比较了Trizol法、Triz01改进法、SDS法、SDS改进法以及柱式植物RNA提取法提取总RNA的效果。结果表明：Trizol法和Trizol改进法所提取的RNA得率很低，且不能有效除去叶片中的多糖成分；SDS改进法能够有效的去除分蘖洋葱叶片中的多糖，提取的RNA中28S RNA亮度约为18SRNA的2倍，OD260／OD280值介于1．7～2．2之间，但RNA的得率偏低，约为56．32／μg／g；柱式植物RNA提取法提取的RNA28S、18S、5S条带清晰，完整性好，RNA的得率最高，为123，58／2μg／g，完全适合于分蘖洋葱进一步的分子生物学研究。

Fresh leaves of tillers onion were used as material, extracted results of total RNA by the method of trizol, modified trizol,SDS, modified SDS and the TIANDZ column plant RNAout were compared. The results showed that trizol method and modified trizol extracted RNA yield was very low, and can not effectively remove the polysaccharide in the leaves. The modified SDS extraction was capable of efficiently removing polysaccharides, the brightness 28S RNA of the RNAs isolated by the modified SDS was two times as much as that of 18S RNA, OD260/OD280 ranged between 1.7-2.2, and the RNA productivities from the leaf of tillering onions was 56. 32 μg/g which was lower than that TIANDZ column plant RNAout. The clear bands, good integrity of 28 S, 18 S, 5 S RNA by the method of TIANDZ column plant RNAout, The RNA productivities was 123. 58 μg/g. It was indicated that the TIANDZ column plant RNAout completely was fit for further biological research.