UV法测定北五加皮的不同时间水提物中绿原酸的含量

Study on the Content of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times by UV

以绿原酸为对照品，利用UV法在UV326nm处进行北五加皮不同时间水提物中绿原酸含量的测定。结果表明：绿原酸在0．0017～0．0101mg／mL的范围内呈良好线性关系（r=0．9992），平均加样回收率为99．1％（n=6，R（RSD=1．09％）。确定水提取北五加皮中绿原酸的最佳时间为2h，该方法简单快速、易于操作，试验结果可靠。

The content of chlorogenic acid in water extract of Cortex periplocae at the different times were determined by UV. Using chlorogenic acid as a standard substance, the content was determined by spectrophotometer at 326 nm. The linear range of rutin were 0. 0017-0. 0101 mg/mL（r=0. 9992）. The average recoveries was 99. 1% （n= 6, RSD= 1.09 %）. Two hours was the best time to extraction of chlorogenic acid. The method was simple, fast, feasible and accurate.