乙肝五项测定结果与HBV DNA的关系及其临床意义

Clinical Significance of Establishing Relationship between the HBsAg Test and HBV DNA

目的探讨乙型肝炎(乙肝)五项结果和HBV DNA检出情况间的关系和临床意义。方法运用聚合酶链反应法检测300例乙肝血清的HBV DNA,并用酶联免疫吸附实验法进行乙肝五项的测定,对结果进行比较分析。结果 300例乙肝门诊的患者中乙肝五项的结果为110例HBsAg(+)、HBeAg(+)、HBcAb(+)(大三阳),其HBV DNA的阳性率为99.1%,85例HBsAg(+)、HBe-Ab(+)、HBcAb(+)(小三阳),其HBV DNA的阳性率为69.4%,39例HBsAg(+)、HBcAb(+),其HBV DNA的阳性率为53.8%,53例HBeAb(+)、HBcAb(+),其HBV DNA的阳性率为35.8%。大三阳和小三阳的HBV DNA阳性率比较差异有统计学意义(χ2=35.406,P<0.05)。结论乙肝五项的结果与HBV DNA的结果有着密切的联系,并且都具备各自的临床意义,因此两者必须结合才能正确地对乙肝患者的病情作出正确分析和判断。

Objective To explore the internal relationship and the clinical significance of HBVM（HBsAg,Anti-HBs,HBeAg,Anti-HBe,Anti-HBc）and HBV DNA（Hepatitis B Virus DNA）.Methods HBVM and HBV-DNA of 300 HBV patients were examined by enzyme linked immunosorbent assay（ELISA）and quantitative fluorescence PCR technique respectively. Results Of all the clinical results of 300 HBV patients,110 patients showed HBsAg（＋）,HBeAg（＋）,HBcAb（＋）（HBV DNA positive rate 99.1%）,85 patients showing HBsAg（＋）,HBeAb（＋）,HBcAb（＋）（HBV DNA positive rate 69.4%）,39 patients showing HBsAg（＋）,HBcAb（＋）（HBV DNA positive rate 53.8%）,53 patients showing HBeAb（＋）,HBcAb（＋）（HBV DNA positive rate 35.8%）.HBV DNA positive rate of HBsAg（＋）,HBeAg（＋）,HBcAb（＋） and HBsAg（＋）,HBeAb（＋）,HBcAb（＋） were different significantly（χ2=35.406,P〈0.05）.Conclusion The results of HBsAg and HBV-DNA are closely related with different clinical meanings,which makes it necessary to combine the two results for a correct diagnosis and treatment.