梅毒三种检测方法的比较

Comparison of the 3 approaches on detection of syphilis

目的比较三种梅毒血清学实验方法的准确性及在临床诊断中的应用价值。方法应用甲苯胺红不加热血清试验(TRUST)、梅毒螺旋体明胶凝集试验(TPPA)、酶联免疫吸附试验(ELISA)检测228例血清样本。结果 TRUST、TPPA、ELISA的敏感性分别为85.83%、98.33%、99.17%;特异性为95.37%、99.07%、98.15%。其他两种方法与敏感性、特异性均较好的TPPA比较,TRUST的敏感性和特异性较低,差异具有有统计学意义(P<0.05);ELISA的敏感性和特异性和TPPA比较,差异无统计学意义(P>0.05)。ELISA与TRUST敏感性比较,差异有统计学意义(P<0.05),ELISA与TRUST特异性比较,差异无统计学意义(P>0.05)。结论 TPPA和ELISA敏感性高、特异性强,是目前用于检测梅毒抗体的首选方法。

Objective To compare the specificity of three kinds of serological approaches on detecting treponema pallidum antibody and the clinical application values in clinic.Methods Three approaches including TRUST,TPPA,ELISA were used to detect 228 serum specimens.Results The sensitivities of the three approaches（TRUST,TPPA,ELISA） were 85.83%,98.33%,99.17%;The specificities of the three approaches were 95.37%,99.07%,98.15% respectively.The sensitivity and specificity of TRUST were lower than those of the TPPA（P0.05）;The sensitivity and specificity of ELISA were not lower than those of the TPPA（P0.05）;The sensitivity of TRUST was lower than that of the ELISA（P0.05）,but the specificity of TRUST was not lower than that of the ELISA（P0.05）.Conclusion It is suggested that TPPA and ELISA are the first choice on detecting syphilis antibody.