实时荧光定量聚合酶链反应检测乙型肝炎病毒DNA室内质控累积数据的评价

Evaluation of cumulated data of indoor quality control for HBV-DNA by FQ-PCR

目的对实时荧光定量聚合酶链反应(PCR)检测乙型肝炎病毒(HBV)DNA室内质控累积的数据进行评价。方法用Taqman实时荧光定量PCR法检测2010年1～12月两个批号的质控血清HBV DNA,计算每次阳性质控结果的常用对数、标准曲线的斜率、截距和相关系数(r)及相关结果的均值(x)、标准差(s)和变异系数(CV)。结果本室2010年测定的室内质控物结果对数的累计数据x±2s范围为4.470～5.429,在参考范围内,符合要求。结论 本实验室采用Taqman实时荧光定量PCR法检测HBV DNA实验中,选用的质控方法和质控品稳定性良好,能可靠有效地为临床提供准确的结果。

Objective To evaluate cumulated data of indoor quality control for HBV-DNA by real-time fluorescent quantitative PCR（FQ-PCR）.Methods Serum HBV DNA from January 2010 to December 2010 was detected by FQ-PCR.Results of positive control mean value（x）,standard deviation（s） and coefficient（r） of variability（CV） of slope rate,intercept and correlation coefficient of standard curve were calculated.Results The logarithm ′s x±2s of cumulated data of indoor quality control for 2010 was all from 4.470-5.429 according with reference range.Conclusion The product and the method are as good as positive indoor quality control for clinic appliance by FQ-PCR.