2Bollwein H, Fuchs I, Koess C. Interrelationship between plas- ma membrane integrity, mitochondrial membrane potential and DNA fragmentation in eryopreserved bovine spermatozoa [J].Reprod Domest Anim,2008,43(2):189-195.
3Perticarari S, Rieei G,Granzotto M, et al. A new multipa- rameter flow cytometric method for human semen analysis [J]. Hum Reprod,2007,22(2) :485-494.
4Christensen P, Boelling D, Pedersen KM, et al. Relation- ship between sperm viability as determined by flow ey- tometry and nonreturn rate of dairy bulls [J]. J Androl,2005,26(1):98-106.
5Agarwal A, Allamaneni SS. Sperm DNA damage assess- ment: a test whose time has come[J]. Fertil Steril, 2005, 84(8) :850-853.
7Riley-Vargas RC, Lanzendorf S, Atkinson JP. Targeted and restricted complement activation on acrosome-reacted spermatozoa[J]. J Clin Invest,2005,115(5) : 1241-1249.
8Petrunkina AM, Gropper B, Gunzel-Apel AR, et al. Func- tional significance of the cell volume for detecting sperm membrane changes and predicting freezability in dog se- men[J]. Reproduction, 2004,128 (6) : 829-842.
9Marchetti C,Jouy N, Leroy-Martin B, et al. Comparison of four fluorochromes for the detection of the inner mito- chondrial membrane potential in human spermatoz- oa and their correlation with sperm motility[J]. Hum Reprod, 2004,19(10) :2267-2276.
6Oosterhuis G J, Mulder A B, Kalsbeek-Batenburg E, et al. Measuring apoptosis in human spermatozoa: a biological assay for semen quality? [J]. Fertil Steril, 2000, 74(2) : 245 -250.
7Ricci G, Perticarari S, Fragonas E, et al. Apoptosis in human sperm: its correlation with semen quality and the presence of leukocytes [ J ]. Hum Reprod, 2002, 17 (10) : 2665 - 2672.
8Vermes I, Haanen C, Steffens-Nakken H, et al. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V[ J ]. J Immunol Methods, 1995, 184(1) : 39 -51.
9黄宇烽,李宏军.检验与临床诊断男科疾病分册.北京:人民军医出版社出版,2007:90-91.
10Len JA, Jenkins JA, Eilts BE, et al. Immediate and delayed ( after cooling) effects of centrifugation on equine sperm. Theriogenology, 2010, 73(2): 225-231.