高分子聚合物对杂交瘤细胞生长的促进作用

Growth promoting effect of polymers on hybridoma cells

为促进杂交瘤细胞在低血清条件下的生长及无血清培养基的开发，通过向培养基中添加一定初始浓度的高分子物质，在批式培养中研究了它们对杂交瘤细胞生长的影响。结果发现了聚乙烯醇（ＰＶＡ）和聚Ｎ－乙烯吡咯烷酮（Ｎ－ＰＶＰ）在低血清浓度下对细胞的生长有促进作用。１０ ｍ ｇ·ｍ Ｌ－ １ 的ＰＶＡ 在１％ ～３％ 血清浓度下，可使最大活细胞密度提高约５０％ ； 与１０ ｍ ｇ·ｍ Ｌ－ １ 胰岛素的作用相当。Ｎ－ＰＶＰ也有相近的结果。对比试验表明， ＰＶＡ 使细胞对葡萄糖的得率提高了８８％ ，揭示了它与胰岛素作用机理的不同； 而Ｎ－ＰＶＰ的存在使台盼蓝不能对死细胞染色。因而推测了聚合物的作用可能和其较强亲水性及结合力有关。试验结果为深入了解生长因子的作用机理，开发低蛋白、低成本的培养基提供了一定的帮助。

To develop low protein medium, the effect of various polymers on hybridoma cell growth was investigated in batch cultures with low serum levels, including PEG, PVA, PVP, Dextran etc. The results show that 10mg·mL -1 PVA (polyvinyl alcohol) increased the maximum viable cell density by up to 50%, the same extent as adding 10mg·mL -1 insulin at serum level of 1%~3%. Similar results were observed when using 10mg·mL -1 N PVP (poly N vinylpyrrolidone). Further experiments indicate that PVA decreased the specific consumption rate of glucose, which implies the growth promoting mechanism of PVA is different from that of insulin. For N PVP, an interesting phenomena observed is that dead cells can not be dyed by trypan blue in the medium containing N PVP. The growth promoting effect of PVA and N PVP is related with their hydropathy and action on cell membrane. These results could be helpful to understanding of the mechanism of growth factors and low protein medium design.