支气管肺炎患儿T淋巴细胞亚群变化情况及临床意义

Study on the changes and significance of the T lymphocytesubtype in children with Bronchial pneumonia

目的研究支气管肺炎患儿外周血T淋巴细胞亚群变化及其临床意义。方法采用免疫荧光流式细胞技术检测了48例支气管肺炎患儿及40例正常儿童外周血T淋巴细胞亚群CD3+T细胞、CD3+CD4+T细胞及CD3+CD8+T细胞水平。结果支气管肺炎患儿CD3+和CD3+CD4+T细胞百分比明显低于正常对照组,分别为(64.12±6.89)%和(70.11±5.09)%(P<0.01)、(26.94±6.24)%和(34.92±6.40)%(P<0.01);而CD3+CD8+T细胞百分比分别为(30.25±6.03)%和(24.47±3.89)%,观察组明显高于对照组(P<0.01)。结论支气管肺炎患儿T淋巴细胞亚群比例改变,提示支气管患儿存在免疫功能异常,为支气管肺炎患儿临床免疫治疗提供理论依据。

Objective To study on the changes and significance of the T lymphocyte subtype in children with Bronchial pneumonia.Methods The flow cytometry（FCM）was used to detected the T lymphocyte subtype in 48 cases of children with Bronchial pneumonia.Results The expression levels of CD3^＋ and CD3^＋CD4^＋ cells in children with Bronchial pneumonia were significantly lower than those of normal controls（NC）,which were（64.12±6.89）% and （70.11±5.09）%（P0.01）,（26.94±6.24）% and （34.92±6.40）%（P0.01）;CD3＋CD8＋ cells （30.25±6.03）% were greatly increased in comparison with NC（24.47±3.89）（P0.01）.Conclusion T lymphocyte subtype can be used to evaluate the immune function of children with Bronchial pneumonia and provide guidance for adoptive immunotherapy.