南方型紫花苜蓿耐盐细胞系的筛选及生理特性分析

Selection and Physiological Characterization of Salt-tolerant Cell Line of Southern Type Alfalfa

以NaCl为选择因子,对长期继代培养的紫花苜蓿愈伤组织进行选择,筛选出耐1.2%NaCl的耐盐细胞系并对其进行生理分析,结果表明:耐盐细胞系对逆境的忍受能力高于对照,积累的脯氨酸和可溶性蛋白含量也高于对照,维持着较高的K+/Na+比,在盐分胁迫下能维持较高的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。

Calluses of Southern type alfalfa cultured for a long term were selected,a cell line tolerant to 1.2% NaCl was isolated by using NaCl as selection pressure.The physiological and biochemical properties of this salt tolerant cell line were analyzed.The results showed that the tolerance of the salt-tolerant cell line was much higher than that of control line.Free proline content and soluble protein content increased in salt-tolerant cell line was higher than that in control,a higher K＋/Na＋ ratio was kept in the salt-tolerant cell line.The activities of superoxide dismutase（SOD）,peroxidase（POD） in salt-tolerant cell line were higher than that of the control under NaCl stress.